Language
English
Publication Date
2-10-2023
Journal
JoVE: Peer Reviewed Scientific Video Journal Article Protocols
DOI
10.3791/64718
PMID
36847377
PMCID
PMC10874126
PubMedCentral® Posted Date
2-17-2024
PubMedCentral® Full Text Version
Author MSS
Abstract
Mouse mammary glands comprise ductal trees, which are lined by epithelial cells and have one opening at the tip of each nipple. The epithelial cells play a major role in mammary gland function and are the origin of most mammary tumors. Introducing genes of interest into mouse mammary epithelial cells is a critical step in evaluating gene function in epithelial cells and generating mouse mammary tumor models. This goal can be accomplished through the intraductal injection of a viral vector carrying the genes of interest into the mouse mammary ductal tree. The injected virus subsequently infects mammary epithelial cells, bringing in the genes of interest. The viral vector can be lentiviral, retroviral, adenoviral, or adenovirus-associated viral (AAV). This study demonstrates how a gene of interest is delivered into mammary epithelial cells through mouse mammary intraductal injection of a viral vector. A lentivirus carrying GFP is used to show stable expression of a delivered gene, and a retrovirus carrying Erbb2 (HER2/Neu) is used to demonstrate oncogene-induced atypical hyperplastic lesions and mammary tumors.
Keywords
Mice, Animals, Mammary Glands, Animal, Epithelial Cells, Gene Transfer Techniques, Oncogenes, Retroviridae, Adenoviridae, Neoplasms, Mammary Neoplasms, Experimental, Mice, Transgenic
Published Open-Access
yes
Recommended Citation
, "In Vivo Gene Delivery into Mouse Mammary Epithelial Cells Through Mammary Intraductal Injection" (2023). Faculty, Staff and Students Publications. 2010.
https://digitalcommons.library.tmc.edu/baylor_docs/2010