Language
English
Publication Date
12-23-2025
Journal
Genome Biology
DOI
10.1186/s13059-025-03923-w
PMID
41437377
PMCID
PMC12723928
PubMedCentral® Posted Date
12-23-2025
PubMedCentral® Full Text Version
Post-print
Abstract
Determining tumor-specificity of MHC-bound peptides is crucial for cancer immunotherapy development, yet current methods struggle with class II peptides and non-reference sequences. We introduce PepQueryMHC, an ultra-fast tool that integrates MHC-bound peptide sequences with translated RNA-seq reads for efficient tumor antigen prioritization. We demonstrate its versatility in prioritizing class I and II tumor antigens, mapping the cellular origins of presented peptides, and resolving uncertainties surrounding the prevalence of proteasome-spliced peptides.
Keywords
Antigens, Neoplasm, Humans, Peptides, Histocompatibility Antigens Class I, Histocompatibility Antigens Class II, Software, Neoplasms, Immunopeptidome, Tumor antigens, Immunoinformatics, Proteogenomics, Major histocompatibility complex
Published Open-Access
yes
Recommended Citation
, "PepQueryMHC: Rapid and Comprehensive Tumor Antigen Prioritization From Immunopeptidomics Data" (2025). Faculty, Staff and Students Publications. 6269.
https://digitalcommons.library.tmc.edu/baylor_docs/6269