Disordered Differentiation and Cellular Senescence in Pediatric Hodgkin Reed-Sternberg Cells

Authors

• Jennifer E Agrusa
• Elmoataz Abdel Fattah
• Howard Lin
• Jessica Velazquez
• Sandra Andrea Salinas
• Harshal A Abhyankar
• Brooks P Scull
• Ryan Fleischmann
• M Tarek Elghetany
• Andrea N Marcogliese
• Choladda V Curry
• Jyotinder N Punia
• Nmazuo W Ozuah
• Olive S Eckstein
• Nader K El-Mallawany
• Nitya Gulati
• Joseph Lubega
• Terzah M Horton
• Kala Y Kamdar
• Kenneth L McClain
• Tsz-Kwong Man
• Carl E Allen

Language

English

Publication Date

4-28-2026

Journal

Blood Advances

DOI

10.1182/bloodadvances.2025016953

PMID

41538304

PMCID

PMC13101695

PubMedCentral® Posted Date

1-19-2026

PubMedCentral® Full Text Version

Post-print

Abstract

Hodgkin lymphoma (HL) is characterized by inflammatory lesions with relatively few (typically < 1%) pathogenic Hodgkin Reed-Sternberg (HRS) cells. The scarcity of malignant HRS cells in HL has historically limited characterization of these disease drivers, and pathogenic mechanisms underlying HL are incompletely defined. Using multiparameter flow cytometry to purify HRS cells and immune cell populations from pediatric HL lesions and control tonsils, we sought to define transcriptional programs in pediatric HRS cells. HRS cells are thought to arise from transformed germinal center B cells. Interestingly, transcriptomic analysis of HRS cells compared to control tonsil CD30+ cells revealed disordered differentiation with multilineage gene expression patterns, and primary HRS cells had distinct transcriptomes compared to HL cell lines. Further, transcriptomic analysis revealed increased expression of senescence-related genes and downregulation of proapoptotic/mitosis-related genes. Gene expression findings were validated by immunohistochemistry and single-cell imaging. In vitro treatment of primary HRS cells with B-cell lymphoma 2 inhibitor, venetoclax, augmented the induction of apoptosis in HRS cells and other cells of the HL immune microenvironment. These findings support the further study of targeting apoptosis resistance as a potential approach to eliminate malignant HRS cells and dismantle inflammatory lesion formation in pediatric HL.

Keywords

Humans, Reed-Sternberg Cells, Hodgkin Disease, Cell Differentiation, Cellular Senescence, Child, Gene Expression Profiling, Apoptosis, Gene Expression Regulation, Neoplastic

Published Open-Access

yes

Recommended Citation

Agrusa, Jennifer E; Abdel Fattah, Elmoataz; Lin, Howard; et al., "Disordered Differentiation and Cellular Senescence in Pediatric Hodgkin Reed-Sternberg Cells" (2026). Faculty, Staff and Students Publications. 7943.
https://digitalcommons.library.tmc.edu/baylor_docs/7943