Language
English
Publication Date
6-19-2026
Journal
STAR Protocols
DOI
10.1016/j.xpro.2026.104547
PMID
42107045
PMCID
PMC13191241
PubMedCentral® Posted Date
5-9-2026
PubMedCentral® Full Text Version
Post-print
Abstract
Isolation and characterization of rare cell types in non-model organisms present a challenge. Here, we provide a protocol to purify specific cells from planaria for downstream RNA sequencing. We employ a one-step dissociation and fixation method that avoids RNA-degrading formaldehyde. We label cells using RNA fluorescence in situ hybridization (RNA FISH) and flow sort to enrich sequencing reads from cells of interest for deeper biological insight.
For complete details on the use and execution of this protocol, please refer to Mann et al.1
Keywords
Animals, In Situ Hybridization, Fluorescence, Planarians, RNA, Sequence Analysis, RNA, Cell Separation, Cell Biology, Developmental biology, Model Organisms, Stem Cells
Published Open-Access
yes
Recommended Citation
McKinney, Mary Cathleen; Morrison, Jason A; Mann, Frederick G; et al., "Protocol for Purifying and Sequencing Cell Subpopulations Based on RNA Fish Signal in Planaria" (2026). Faculty, Staff and Students Publications. 8011.
https://digitalcommons.library.tmc.edu/baylor_docs/8011