Protocol for Lentiviral Generation and Transduction of Human-Derived Endometrial Epithelial Organoids To Study Uterine Function and Pathologies

Authors

• Sydney E Parks
• Diana Monsivais

Language

English

Publication Date

6-19-2026

Journal

STAR Protocols

DOI

OI: 10.1016/j.xpro.2026.104558

PMID

42101936

PMCID

PMC13185981

PubMedCentral® Posted Date

5-7-2026

PubMedCentral® Full Text Version

Post-print

Abstract

The use of patient-derived endometrial epithelial organoids has revolutionized the field of uterine biology. Here, we present a protocol for generating lentivirus and transducing human-derived endometrial epithelial organoids for the study of endometrial function and pathologies. We describe steps for lentiviral production, lentiviral transduction of endometrial epithelial organoids, and endometrial epithelial organoid passaging. We then detail procedures for validating lentiviral transduction. This protocol allows for knocking down or overexpressing genes of interest, enabling investigation of specific targets in a patient-centric model.

Keywords

Humans, Female, Lentivirus, Organoids, Endometrium, Transduction, Genetic, Epithelial Cells, Uterus, Genetic Vectors, Cell Culture Techniques, Cell Biology, Cell culture, Gene Expression, Organoids

Published Open-Access

yes

Recommended Citation

Sydney E Parks and Diana Monsivais, "Protocol for Lentiviral Generation and Transduction of Human-Derived Endometrial Epithelial Organoids To Study Uterine Function and Pathologies" (2026). Faculty, Staff and Students Publications. 8180.
https://digitalcommons.library.tmc.edu/baylor_docs/8180