Language
English
Publication Date
9-5-2024
Journal
bioRxiv
DOI
10.1101/2023.05.30.542907
PMID
39282326
PMCID
PMC11398385
Abstract
Background: Human noroviruses are a leading cause of acute and sporadic gastroenteritis worldwide. The evolution of human noroviruses in immunocompromised persons has been evaluated in many studies. Much less is known about the evolutionary dynamics of human norovirus in healthy adults.
Methods: We used sequential samples collected from a controlled human infection study with GI.1/Norwalk/US/68 virus to evaluate intra- and inter-host evolution of a human norovirus in healthy adults. Up to 12 samples from day 1 to day 56 post-challenge were sequenced using a norovirus-specific capture probe method.
Results: Complete genomes were assembled, even in samples that were below the limit of detection of standard RT-qPCR assays, up to 28 days post-challenge. Analysis of 123 complete genomes showed changes in the GI.1 genome in all persons, but there were no conserved changes across all persons. Single nucleotide variants resulting in non-synonymous amino acid changes were observed in all proteins, with the capsid VP1 and nonstructural protein NS3 having the largest numbers of changes.
Conclusions: These data highlight the potential of a new capture-based sequencing approach to assemble human norovirus genomes with high sensitivity and demonstrate limited conserved immune pressure-driven evolution of GI.1 virus in healthy adults.
Keywords
Human norovirus, Norwalk virus, Oligonucleotide capture sequencing, Full length genome, Virus evolution, Controlled human infection model
Published Open-Access
yes
Recommended Citation
Ramani, Sasirekha; Javornik Cregeen, Sara J; Surathu, Anil; et al., "Intra- and Inter-Host Evolution of Human Norovirus in Healthy Adults" (2024). Faculty, Staff and Students Publications. 8343.
https://digitalcommons.library.tmc.edu/baylor_docs/8343