Language
English
Publication Date
7-15-2026
Journal
Biology Open
DOI
10.1242/bio.062386
PMID
42549485
PMCID
PMC13488407
PubMedCentral® Posted Date
8-4-2026
PubMedCentral® Full Text Version
Post-print
Abstract
The Fucci mouse lines are valuable tools for tracing cell cycle stages during development and in postnatal tissues; however, the insertion loci for the transgenes remain unidentified. Here, we report the genomic insertion sites of both the Fucci-G1 and -S/G2/M transgenes, identified through whole-genome sequencing. Interestingly, the Fucci-G1 transgene is located proximal to the Shh lung gut enhancer (SLGE) on chromosome 5. Homozygous Fucci-G1 embryos are perinatal lethal but survive until embryonic day (E)18.5, exhibiting craniofacial and foregut-related defects. Expression of Shh and canonical targets are downregulated in the pharyngeal arches of E11.5 homozygous Fucci-G1 embryos. Furthermore, in situ hybridization analysis showed diminished Shh expression in the pharyngeal and foregut endoderm, consistent with disruptions to SLGE activity. Our results indicate that heterozygous Fucci-G1 mice are suitable for cell cycle studies; however, we caution potential genetic interference when investigating SHH-related pathways.
Keywords
Animals, Hedgehog Proteins, Mice, Gene Expression Regulation, Developmental, Mice, Transgenic, Mutagenesis, Insertional, Transgenes, Signal Transduction, Fucci, Shh, Hedgehog signaling, Transgenic mouse model, Genotyping
Published Open-Access
yes
Recommended Citation
Morikawa, Yuka; Bromley, Ann; Steimle, Jeffrey D; et al., "Fucci-G1 Insertion Partially Disrupts Shh Expression" (2026). Faculty, Staff and Students Publications. 8600.
https://digitalcommons.library.tmc.edu/baylor_docs/8600