Language
English
Publication Date
1-7-2025
Journal
Microbiology Spectrum
DOI
10.1128/spectrum.00998-24
PMID
39656008
PMCID
PMC11705802
PubMedCentral® Posted Date
12-10-2024
PubMedCentral® Full Text Version
Post-print
Abstract
This study compared intestinal DNA phage dynamics and gut microbiota changes observed at the onset of coronavirus disease 2019 (COVID-19). The study participants included 19 healthy individuals and 19 patients with severe acute respiratory syndrome coronavirus 2 infection. Significant differences were observed in the diversity of the intestinal DNA virome after the onset of COVID-19 compared with that in healthy individuals. Classification by their tail morphology resulted in the order Caudovirales, a double-stranded DNA phage, accounting for >95% of all participants. In classifying phages based on host bacteria, a decreased number of phages infecting mainly the Clostridia class was observed immediately after the onset of COVID-19 and recovered over time. After the onset of COVID-19, two distinct movement patterns of intestinal phages and their host bacteria were observed: phage- and bacteria-predominant. The abundance of obligate anaerobes, such as Clostridium_sense_strict_1, Fusicatenibacter, and Romboutsia, and the phages hosting these bacteria decreased immediately after the onset of COVID-19, and faster phage recovery was observed compared with bacterial recovery. In contrast, the genus Staphylococcus, a facultative anaerobic bacterium, increased immediately after the onset of COVID-19, whereas the phages infecting Staphylococcus decreased. Furthermore, immediately after the onset of COVID-19, the percentage of lytic phages increased, whereas that of temperate phages decreased. These observations suggest that the gut microbiota dysbiosis observed immediately after the onset of COVID-19 may be linked to phage dynamics that control gut microbiota and may also affect the recovery from dysbiosis.
Keywords
Humans, Gastrointestinal Microbiome, Dysbiosis, COVID-19, Bacteriophages, SARS-CoV-2, Adult, Bacteria, Female, Male, Middle Aged, Lysogeny, Virome, Aged, Feces, Intestines, DNA, Viral, COVID-19, SARS-CoV-2, gut microbiome, bacteriophages
Published Open-Access
yes
Recommended Citation
Ishizaka, Aya; Tamura, Azumi; Koga, Michiko; et al., "Dysbiosis of Gut Microbiota in COVID-19 Is Associated With Intestinal Dna Phage Dynamics of Lysogenic and Lytic Infection" (2025). Faculty, Staff and Students Publications. 8737.
https://digitalcommons.library.tmc.edu/baylor_docs/8737