Language

English

Publication Date

8-1-2025

Journal

Journal of Visualized Experiments

DOI

10.3791/68472

PMID

40824831

PMCID

PMC13276241

PubMedCentral® Posted Date

6-19-2026

PubMedCentral® Full Text Version

Author MSS

Abstract

The murine olfactory epithelium is the initial entry point of the olfactory system, housing various cell types that include olfactory sensory neurons, their regenerating progenitors, and support cells. Olfactory sensory neurons transduce chemical odorants into neural signals, yet the mechanisms underlying how these cells develop and turnover, create synapses with the olfactory bulb, and regulate their odorant receptors remain areas of intense study. Located on the dorsal aspect of the nasal cavity, the olfactory epithelium adheres to intricate bony structures known as turbinates. This anatomy poses unique challenges for its extraction and dissociation, especially in the context of preparing viable single-cell suspensions. Because single-cell suspension protocols often involve preparatory steps (e.g., papain dissociation, FACS) that can stress cell viability and/or delay library preparation, minimizing tissue extraction time is crucial. This article presents a streamlined method for the rapid dissection of the olfactory epithelium and a protocol for generating high-quality single-nucleus suspensions.

Keywords

Animals, Mice, Olfactory Mucosa, Dissection, Cell Nucleus

Published Open-Access

yes

Download

Share

COinS
 
 

To view the content in your browser, please download Adobe Reader or, alternately,
you may Download the file to your hard drive.

NOTE: The latest versions of Adobe Reader do not support viewing PDF files within Firefox on Mac OS and if you are using a modern (Intel) Mac, there is no official plugin for viewing PDF files within the browser window.