Date of Graduation

12-2009

Program Affiliation

Biomedical Sciences

Degree Name

Masters of Science (MS)

Advisor/Committee Chair

Cheng Chi Lee

Committee Member

Michael Blackburn

Committee Member

William Dubinsky

Committee Member

Henry Strobel

Committee Member

Rodney Kellems

Abstract

Our laboratory’s ability to induce deep hypometabolism (DH) via an injection of adenosine monophosphate (5’-AMP) followed by placement in an ambient temperature (Ta) around 15oC currently can last about 3-9 hours. While we have insight into how 5’-AMP induced hypometabolism is initiated, it remains unclear how arousal from hypometabolism is controlled. Other laboratory members have been unable to prolong this process safely and effectively with previous attempts of re-injecting a dose of 5’-AMP upon arousal or by decreasing the Ta. While these methods worked in suppressing arousal, the mortality rate is also increased. To gather a better understanding of the process, a metabolic panel from serum samples was run giving insight into possible biochemical events occurring from euthermia to DH to spontaneous arousal and back to euthermia. Analyzing this data revealed many changes in metabolites. A few metabolites that stood out such as glucose and pyruvate did not alter the outcome in arousal rate over saline injection. Additional metabolite analysis revealed that an amino acid, arginine, was significantly reduced during DH. This amino acid seems to be a key component in prolonging our process possibly due to its intricate role in the urea cycle. Supplemental arginine is clearly more beneficial both in suppressing arousal and increasing the survival rate after 12 hours in DH.

Keywords

hypometabolism, 5’-AMP, Suspended Animation, urea cycle, Metabolomics, energy utilization, hibernation, purine metabolism, mouse, hypothermia

Included in

Biochemistry Commons

Share

COinS