FGL2-Targeting T Cells Exhibit Antitumor Effects on Glioblastoma and Recruit Tumor-Specific Brain-Resident Memory T Cells

Authors

Qingnan Zhao
Jiemiao Hu
Lingyuan Kong
Shan Jiang
Xiangjun Tian
Jing Wang
Rintaro Hashizume
Zhiliang Jia
Natalie Wall Fowlkes
Jun Yan
Xueqing Xia
Sofia F Yi
Long Hoang Dao
David Masopust
Amy B Heimberger
Shulin Li

Publication Date

2-10-2023

Journal

Nature Communications

Abstract

Although tissue-resident memory T (TRM) cells specific for previously encountered pathogens have been characterized, the induction and recruitment of brain TRM cells following immune therapy has not been observed in the context of glioblastoma. Here, we show that T cells expressing fibrinogen-like 2 (FGL2)–specific single-chain variable fragments (T-αFGL2) can induce tumor-specific CD8+ TRM cells that prevent glioblastoma recurrence. These CD8+ TRM cells display a highly expanded T cell receptor repertoire distinct from that found in peripheral tissue. When adoptively transferred to the brains of either immunocompetent or T cell-deficient naïve mice, these CD8+ TRM cells reject glioma cells. Mechanistically, T-αFGL2 cell treatment increased the number of CD69+CD8+ brain-resident memory T cells in tumor-bearing mice via a CXCL9/10 and CXCR3 chemokine axis. These findings suggest that tumor-specific brain-resident CD8+ TRM cells may have promising implications for the prevention of brain tumor recurrence.

Keywords

Animals, Mice, Brain, CD8-Positive T-Lymphocytes, Glioblastoma, Immunologic Memory, Memory T Cells, Neoplasm Recurrence, Local, T-Lymphocytes, Cancer immunotherapy, CNS cancer, T cells, Tumour immunology

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Supplementary Materials

PMID: 36759517