Publication Date
2-21-2024
Journal
British Journal of Ophtalmology
Abstract
PURPOSE: Corneal biomechanical failure is the hallmark of keratoconus (KC); however, the cause of this failure remains elusive. Collagen type XII (
METHODS: TaqMan quantitative PCR was performed on 31 corneal epithelium samples of progressive KC and myopic control eyes. Tissue microarrays were constructed using full-thickness corneas from 61 KC cases during keratoplasty and 18 non-KC autopsy eyes and stained with an antibody specific to COL12A1. Additionally,
RESULTS: COL12A1 expression was reduced at transcript levels in KC epithelium compared to controls (ratio: 0.58, p<0.03). Immunohistochemical studies demonstrated that COL12A1 protein expression in BL was undetectable, with reduced expression in KC epithelium, basement membrane, and stroma.
CONCLUSIONS: The apparent absence of COL12A1 in KC BL, together with the functional importance that
Keywords
Humans, Keratoconus, Collagen Type XII, HEK293 Cells, Cornea, Epithelium, Corneal
Included in
Eye Diseases Commons, Laboratory Medicine Commons, Medical Pathology Commons, Ophthalmology Commons
Comments
PMID: 36746614