Study of polytopic membrane protein topological organization as a function of membrane lipid composition.
Methods Mol Biol. 2010; 619: 79–101.
A protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (SCAM). SCAM is adapted to follow changes in membrane protein topology as a function of changes in membrane lipid composition. The strategy described can be adapted to any membrane system.
Bacterial Proteins, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Glycosyltransferases, Immunoprecipitation, Membrane Lipids, Membrane Proteins, Membrane Transport Proteins, Phosphatidylethanolamines, Phospholipids