Publication Date
4-1-2024
Journal
Biomedical Optics Express
Abstract
To improve particle radiotherapy, we need a better understanding of the biology of radiation effects, particularly in heavy ion radiation therapy, where global responses are observed despite energy deposition in only a subset of cells. Here, we integrated a high-speed swept confocally-aligned planar excitation (SCAPE) microscope into a focused ion beam irradiation platform to allow real-time 3D structural and functional imaging of living biological samples during and after irradiation. We demonstrate dynamic imaging of the acute effects of irradiation on 3D cultures of U87 human glioblastoma cells, revealing characteristic changes in cellular movement and intracellular calcium signaling following ionizing irradiation.
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Biomedical Informatics Commons, Eye Diseases Commons, Medical Biochemistry Commons, Medical Molecular Biology Commons, Oncology Commons, Ophthalmology Commons
Comments
PMID: 38633084