Aba-Mediated Regulation of Rice Grain Quality and Seed Dormancy Via The Nf-Yb1-Slrl2-Bhlh144 Module

Authors

Jin-Dong Wang
Jing Wang
Li-Chun Huang
Li-Jun Kan
Chu-Xin Wang
Min Xiong
Peng Zhou
Li-Hui Zhou
Chen Chen
Dong-Sheng Zhao
Xiao-Lei Fan
Chang-Quan Zhang
Yong Zhou
Lin Zhang
Qiao-Quan Liu
Qian-Feng Li

Publication Date

5-27-2024

Journal

Nature Communications

Abstract

Abscisic acid (ABA) plays a crucial role in promoting plant stress resistance and seed dormancy. However, how ABA regulates rice quality remains unclear. This study identifies a key transcription factor SLR1-like2 (SLRL2), which mediates the ABA-regulated amylose content (AC) of rice. Mechanistically, SLRL2 interacts with NF-YB1 to co-regulate Wx, a determinant of AC and rice quality. In contrast to SLR1, SLRL2 is ABA inducible but insensitive to GA. In addition, SLRL2 exhibits DNA-binding activity and directly regulates the expression of Wx, bHLH144 and MFT2. SLRL2 competes with NF-YC12 for interaction with NF-YB1. NF-YB1 also directly represses SLRL2 transcription. Genetic validation supports that SLRL2 functions downstream of NF-YB1 and bHLH144 in regulating rice AC. Thus, an NF-YB1-SLRL2-bHLH144 regulatory module is successfully revealed. Furthermore, SLRL2 regulates rice dormancy by modulating the expression of MFT2. In conclusion, this study revealed an ABA-responsive regulatory cascade that functions in both rice quality and seed dormancy.

Keywords

Oryza, Abscisic Acid, Plant Proteins, Gene Expression Regulation, Plant, Plant Dormancy, Transcription Factors, CCAAT-Binding Factor, Seeds, Basic Helix-Loop-Helix Transcription Factors, Amylose, Edible Grain, Plants, Genetically Modified, Plant molecular biology, Agricultural genetics, Plant signalling

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Associated Data

DOI

10.1038/s41467-024-48760-w

PMID

38802342

PMCID

PMC11130328

PubMedCentral® Posted Date

5-24-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes