Rationale for reading fluconazole MICs at 24 hours rather than 48 hours when testing Candida spp. by the CLSI M27-A2 standard method.

Publication Date



Antimicrob Agents Chemother. 2008 November; 52(11): 4175–4177.


We investigated if CLSI M27-A2 Candida species breakpoints for fluconazole MIC are valid when read at 24 h. Analysis of a data set showed good correlation between 48- and 24-h MICs, as well as similar outcomes and pharmacodynamic efficacy parameters, except for isolates in the susceptible dose-dependent category, such as Candida glabrata.


Animals, Antifungal Agents, Candida, Candidiasis, Databases, Factual, Fluconazole, Humans, Microbial Sensitivity Tests, Models, Biological, Time Factors