Publication Date
6-21-2024
Journal
STAR Protocols
DOI
10.1016/j.xpro.2024.103078
PMID
38781075
PMCID
PMC11141250
PubMedCentral® Posted Date
5-21-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Humans, Brain Neoplasms, Animals, Mice, Xenograft Model Antitumor Assays, Immunotherapy, Adoptive, T-Lymphocytes, Leukocytes, Mononuclear, Receptors, Chimeric Antigen
Abstract
Here, we present a protocol for preclinical evaluation of locoregionally delivered CAR T cells in patient-derived xenograft models of primary, metastatic, and recurrent brain tumors. We provide instructions for isolating peripheral blood mononuclear cells (PBMCs), producing CAR T cells in conjunction with locoregional delivery, and preclinical trial design and analysis involving CAR T cells. Additionally, we describe comprehensive preclinical readouts and guidelines for critical endpoint sample collections. In line with clinical trial procedures, our protocol broadens available treatment modalities for direct clinical translation. For complete details on the use and execution of this protocol, please refer to Donovan et al1.