Parallel Use of Low-Complexity Automated Nucleic Acid Amplification Tests on Respiratory Samples and Stool with or Without Lateral Flow Lipoarabinomannan Assays to Detect Pulmonary Tuberculosis Disease in Children

Authors

Laura Olbrich
Alexander W Kay
Stephanie Bjerrum
Bada Yang
Johanna Åhsberg
Ruvandhi R Nathavitharana
Andreas Lundh
Maunank Shah
Devan Jaganath

Publication Date

5-13-2024

Journal

Cochrane Database of Systematic Reviews

DOI

10.1002/14651858.CD016071

PMID

39908066

PMCID

PMC11089514

PubMedCentral® Posted Date

5-13-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Humans, Nucleic Acid Amplification Techniques, Lipopolysaccharides, Tuberculosis, Pulmonary, Child, Feces, HIV Infections, Sensitivity and Specificity, Sputum, Mycobacterium tuberculosis

Abstract

This is a protocol for a Cochrane Review (diagnostic). The objectives are as follows: To compare the diagnostic accuracy of the parallel use of low-complexity automated nucleic acid amplification tests on respiratory and stool specimens in children and lateral flow urine lipoarabinomannan amongst children with HIV versus each assay alone for detecting pulmonary tuberculosis. Secondary objectives To compare the diagnostic accuracy of low-complexity automated nucleic acid amplification tests on respiratory and stool specimens in combination versus each sample type alone. To investigate the following sources of heterogeneity: clinical setting, signs and symptoms of pulmonary tuberculosis disease, screening positivity by chest X-ray abnormalities, age group, specimen type; and also amongst children with HIV: CD4 cell-count or percent category, advanced HIV disease, and serious illness.