Publication Date
6-11-2024
Journal
Stem Cell Reports
DOI
10.1016/j.stemcr.2024.04.008
PMID
38759644
PMCID
PMC11297560
PubMedCentral® Posted Date
5-16-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Keywords
Humans, Organoids, Brain, Reproducibility of Results, Prosencephalon
Abstract
Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.