Analysis of Variable Major Protein Antigenic Variation in the Relapsing Fever Spirochete, Borrelia miyamotoi, in Response to Polyclonal Antibody Selection Pressure

Authors

Robert D Gilmore
Brittany A Armstrong
Kevin S Brandt
Taylor J Van Gundy
Andrias Hojgaard
Job E Lopez
Alexander R Kneubehl

Publication Date

1-1-2023

Journal

PLoS One

DOI

10.1371/journal.pone.0281942

PMID

36827340

PMCID

PMC9955969

PubMedCentral® Posted Date

2-24-2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Animals, Mice, Relapsing Fever, Borrelia, Ticks, Antibodies, Antigenic Variation, Ixodes

Abstract

Borrelia miyamotoi is a tick-transmitted spirochete that is genetically grouped with relapsing fever Borrelia and possesses multiple archived pseudogenes that encode variable major proteins (Vmps). Vmps are divided into two groups based on molecular size; variable large proteins (Vlps) and variable small proteins (Vsps). Relapsing fever Borrelia undergo Vmp gene conversion at a single expression locus to generate new serotypes by antigenic switching which is the basis for immune evasion that causes relapsing fever in patients. This study focused on B. miyamotoi vmp expression when spirochetes were subjected to antibody killing selection pressure. We incubated a low passage parent strain with mouse anti-B. miyamotoi polyclonal antiserum which killed the majority population, however, antibody-resistant reisolates were recovered. PCR analysis of the gene expression locus in the reisolates showed vsp1 was replaced by Vlp-encoded genes. Gel electrophoresis protein profiles and immunoblots of the reisolates revealed additional Vlps indicating that new serotype populations were selected by antibody pressure. Sequencing of amplicons from the expression locus of the reisolates confirmed the presence of a predominant majority serotype population with minority variants. These findings confirm previous work demonstrating gene conversion in B. miyamotoi and that multiple serotype populations expressing different vmps arise when subjected to antibody selection. The findings also provide evidence for spontaneous serotype variation emerging from culture growth in the absence of antibody pressure. Validation and determination of the type, number, and frequency of serotype variants that arise during animal infections await further investigations.