Colonization of Patients Hospitalized at Orthopedic Department of Tertiary Hospital in Uganda With Extended-Spectrum Beta-Lactamase-Producing Enterobacterales

Authors

Jules Bizimana
Jerome Ndayisenga
Henry Kajumbura
Phillip Mulepo
Najjuka Florence Christine

Publication Date

4-1-2023

Journal

Antimicrobial Resistance & Infection Control

DOI

10.1186/s13756-023-01229-9

PMID

37005681

PMCID

PMC10068153

PubMedCentral® Posted Date

4-1-2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Humans, Adolescent, Enterobacteriaceae Infections, Enterobacteriaceae, Tertiary Care Centers, Uganda, Anti-Bacterial Agents, beta-Lactamases, Gammaproteobacteria, Colonization of patients, Orthopedic Department, Mulago Referral Hospital, Extended-spectrum beta-lactamase producing Enterobacterales (ESBL-PE)

Abstract

BACKGROUND: Beta-lactamase production remains the most contributing factor to beta-lactam resistance. Extended-Spectrum Beta-Lactamase-Producing Enterobacterales (ESBL-PE) are associated with risk factors both in hospital and community settings.

OBJECTIVES: To assess the incidence and risk factors for intestinal carriage of ESBL-PE among patients admitted to orthopedic ward of Mulago National Referral Hospital, and to analyze the acquisition of ESBL-PE during hospital stay and associated factors.

METHODS: We screened 172 patients aged 18 years old and above who got admitted to the orthopedic ward of Mulago National Referral Hospital between May to July 2017. Stool samples or rectal swabs were collected at admission, every 3 days until fourteen days and screened for ESBL-PE. Data on demographic status, antibiotic use, admission and travel, length of hospital stay, hygiene practices and drinking boiled water were analyzed by logistic regression and cox regression model.

RESULTS: At admission, 61% of patients showed intestinal ESBL-PE carriage. Co- resistance was common but no Carbapenem resistance was detected. Of the ESBL-PE negative, 49% were colonized during hospitalization. On admission, prior antibiotic use was significantly associated with carriage, but none was associated with acquisition during hospitalization at p-value < 0.05.

CONCLUSION: Carriage of ESBL-PE on admissions and acquisition at orthopedic ward of Mulago Hospital were high, and dissemination into the community are of substantial concern. We suggested refinement of empirical treatment based on risk stratification, and enhanced infection control measures that target health care workers, patients and attendants.