Mammalian Snm1 participates in cellular responses to genotoxic and mitotic stress

Christopher Thomas Richie, The University of Texas Graduate School of Biomedical Sciences at Houston


Eukaryotic cells have evolved a complex network of metabolic processes and regulatory systems to help ensure that hereditary information is protected or restored when exposed to genotoxic agents. Two members of the Snm1 protein family have been characterized; scSNM1/PSO2, a yeast gene responsible for repair of DNA interstrand crosslinks, and hARTEMIS, a human gene that is mutated in radiosensitive severe combined immunodeficiency (RS-SCID). Here we report on another member of this protein family, hSNM1, and its response to DNA damage and mitotic stress. We have found that this protein colocalizes and physically associates with 53BP1, a crucial member of the mammalian response to DNA damage. In addition, hSnm1 interacts with several proteins involved in mitosis, and mSNM1 deficiency causes a mitotic checkpoint defect in mouse embryonic fibroblasts.

Subject Area

Molecular biology

Recommended Citation

Richie, Christopher Thomas, "Mammalian Snm1 participates in cellular responses to genotoxic and mitotic stress" (2003). Texas Medical Center Dissertations (via ProQuest). AAI3083501.