Whole genome analysis of Fusobacterium nucleatum, Rickettsia typhi and Francisella tularensis

Sandor Ervin Karpathy, The University of Texas Graduate School of Biomedical Sciences at Houston


The genomes of Fusobacterium nucleatum subspecies polymorphum strain ATCC 10953, Rickettsia typhi strain Wilmington, and Francisella tularensis subspecies holarctica strain OSU18 were sequenced, annotated, and analyzed. Each genome was then compared to the sequenced genomes of closely related bacteria. The genome of F. nucleatum ATCC 10953 was compared to two additional F. nucleatum subspecies, subspecies nucleatum and subspecies vincentii. This analysis revealed substantial evidence of horizontal gene transfer along with considerable genetic diversity within the species of F. nucleatum. R. typhi was compared to R. prowazekii and R. conorii. This analysis uncovered a hotspot for chromosomal rearrangements in the Spotted Fever Group but not the Typhus Group Rickettsia and revealed the close genetic relationship between the Typhus Group rickettsial species. F. tularensis OSU18 was compared to two additional F. tularensis strains. These comparisons uncovered significant chromosomal rearrangements between F. tularensis subspecies due to recombination between insertion sequence elements.

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Recommended Citation

Karpathy, Sandor Ervin, "Whole genome analysis of Fusobacterium nucleatum, Rickettsia typhi and Francisella tularensis" (2006). Texas Medical Center Dissertations (via ProQuest). AAI3209530.