Prematurity Blunts the Insulin- and Amino Acid-Induced Stimulation of Translation Initiation and Protein Synthesis in Skeletal Muscle of Neonatal Pigs

Authors

Marko Rudar
Jane K Naberhuis
Agus Suryawan
Hanh V Nguyen
Barbara Stoll
Candace C Style
Mariatu A Verla
Oluyinka O Olutoye
Douglas G Burrin
Marta L Fiorotto
Teresa A Davis

Publication Date

3-1-2021

Journal

American Journal of Physiology-Endocrinology and Metabolism

DOI

10.1152/ajpendo.00203.2020

PMID

33427053

PMCID

PMC7988778

PubMedCentral® Posted Date

1-11-2021

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Amino Acids, Animals, Animals, Newborn, Female, Insulin, Mechanistic Target of Rapamycin Complex 1, Muscle, Skeletal, Peptide Chain Initiation, Translational, Pregnancy, Premature Birth, Protein Biosynthesis, Signal Transduction, Swine, amino acid, insulin, mTOR, prematurity, protein synthesis

Abstract

Extrauterine growth restriction in premature infants is largely attributed to reduced lean mass accretion and is associated with long-term morbidities. Previously, we demonstrated that prematurity blunts the feeding-induced stimulation of translation initiation signaling and protein synthesis in skeletal muscle of neonatal pigs. The objective of the current study was to determine whether the blunted feeding response is mediated by reduced responsiveness to insulin, amino acids, or both. Pigs delivered by cesarean section preterm (PT; 103 days,