Gene Expression Analysis of Environmental Temperature and High-Fat Diet-Induced Changes in Mouse Supraclavicular Brown Adipose Tissue

Authors

Yufeng Shi
Honglei Zhai
Sharon John
Yi-Ting Shen
Yali Ran
Giang Hoang
Miao-Hsueh Chen

Publication Date

6-2-2021

Journal

Cells

DOI

10.3390/cells10061370

PMID

34199472

PMCID

PMC8226907

PubMedCentral® Posted Date

6-2-2021

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

Keywords

Adipose Tissue, Brown, Animals, Diet, High-Fat, Gene Expression Profiling, Gene Expression Regulation, Humans, Mice, Thermogenesis, Uncoupling Protein 1, adipose tissue, supraclavicular brown adipose tissue, thermogenesis, thermoneutrality, high-fat diet

Abstract

Obesity, a dysregulation of adipose tissue, is a major health risk factor associated with many diseases. Brown adipose tissue (BAT)-mediated thermogenesis can potentially regulate energy expenditure, making it an attractive therapeutic target to combat obesity. Here, we characterize the effects of cold exposure, thermoneutrality, and high-fat diet (HFD) feeding on mouse supraclavicular BAT (scBAT) morphology and BAT-associated gene expression compared to other adipose depots, including the interscapular BAT (iBAT). scBAT was as sensitive to cold induced thermogenesis as iBAT and showed reduced thermogenic effect under thermoneutrality. While both scBAT and iBAT are sensitive to cold, the expression of genes involved in nutrient processing is different. The scBAT also showed less depot weight gain and more single-lipid adipocytes, while the expression of BAT thermogenic genes, such as