Faculty, Staff and Student Publications

Publication Date

12-11-2024

Journal

International Journal of Molecular Sciences

Abstract

Periodontitis disproportionately affects genetic ancestral/ethnic groups. To characterize the oral microbiome from different genetic ancestral/ethnic groups, we collected 161 dental plaque samples from self-identified African Americans (AAs), Caucasian Americans (CAs), and Hispanic Americans (HAs) with clinical gingival health or biofilm-induced gingivitis on an intact periodontium. DNA was extracted from these samples, and then DNA libraries were prepared and sequenced using an Illumina NovaSeq high-throughput sequencer. We found significant differences in the diversity and abundance of microbial taxa among dental plaque samples of the AA, CA, and HA groups. We also identified unique microbial species in a self-reported ancestral/ethnic group. Moreover, we revealed variations in functional potentials of the oral microbiome among the three ancestral/ethnic groups, with greater diversity and abundance of antibiotic-resistant genes in the oral microbiome and significantly more genes involved in the modification of glycoconjugates and oligo- and polysaccharides in AAs than in CAs and HAs. Our observations suggest that the variations in the oral microbiome associated with ancestral/ethnic backgrounds may directly relate to their virulence potential including their abilities to induce host immune responses and to resist antibiotic treatment. These finding can be a steppingstone for developing precision medicine and personalized periodontal prevention/treatment and for reducing oral health disparities.

Keywords

Adult, Female, Humans, Male, Middle Aged, Bacteria, Biofilms, Black or African American, Dental Plaque, Ethnicity, Gingivitis, Hispanic or Latino, Microbiota, Mouth, Periodontitis, RNA, Ribosomal, 16S, Self Report, White, oral microbiome health disparities, periodontitis

DOI

10.3390/ijms252413303

PMID

39769067

PMCID

PMC11677810

PubMedCentral® Posted Date

12-11-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes

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