Micro-computed Tomography Assessment of Bone Structure in Aging Mice

Authors

Junbo Shim
Chihiro Iwaya
Catherine G Ambrose
Akiko Suzuki
Junichi Iwata

Publication Date

5-17-2022

Journal

Scientific Reports

Abstract

High-resolution computed tomography (CT) is widely used to assess bone structure under physiological and pathological conditions. Although the analytic protocols and parameters for micro-CT (μCT) analyses in mice are standardized for long bones, vertebrae, and the palms in aging mice, they have not yet been established for craniofacial bones. In this study, we conducted a morphometric assessment of craniofacial bones, in comparison with long bones, in aging mice. Although age-related changes were observed in the microarchitecture of the femur, tibia, vertebra, and basisphenoid bone, and were more pronounced in females than in males, the microarchitecture of both the interparietal bone and body of the mandible, which develop by intramembranous ossification, was less affected by age and sex. By contrast, the condyle of the mandible was more affected by aging in males compared to females. Taken together, our results indicate that mouse craniofacial bones are uniquely affected by age and sex.

Keywords

Aging, Animals, Bone Density, Female, Femur, Male, Mice, Skull, X-Ray Microtomography

DOI

10.1038/s41598-022-11965-4

PMID

35581227

PMCID

PMC9114112

PubMedCentral® Posted Date

5-17-2022

PubMedCentral® Full Text Version

Post-print

Recommended Citation

Shim, Junbo; Iwaya, Chihiro; Ambrose, Catherine G; Suzuki, Akiko; and Iwata, Junichi, "Micro-computed Tomography Assessment of Bone Structure in Aging Mice" (2022). Faculty, Staff and Student Publications. 78.
https://digitalcommons.library.tmc.edu/uthdb_docs/78

Published Open-Access

yes