Student and Faculty Publications
Publication Date
1-1-2024
Journal
Journal of Veterinary Internal Medicine
Abstract
BACKGROUND: Pancreatitis is an important cause of disease and death in dogs. Available circulating biomarkers are not sufficiently sensitive and specific for a definitive diagnosis.
HYPOTHESIS: Circulating microRNAs would be differentially expressed in dogs with chronic pancreatitis and could have potential as diagnostic biomarkers.
ANIMALS: Healthy controls (n = 19) and dogs with naturally occurring pancreatitis (n = 17).
METHODS: A retrospective case-control study. Dogs with pancreatitis were included if they satisfied diagnostic criteria for pancreatitis as adjudicated by 3 experts. MicroRNA was extracted from stored serum samples and sequenced. Reads were mapped to mature microRNA sequences in the canine, mouse, and human genomes. Differentially expressed microRNAs were identified and the potential mechanistic relevance explored using Qiagen Ingenuity Pathway Analysis (IPA).
RESULTS: Reads mapping to 196 mature microRNA sequences were detected. Eight circulating microRNAs were significantly differentially expressed in dogs with pancreatitis (≥2-fold change and false discovery rate
CONCLUSIONS: The significantly differentially expressed microRNAs represent promising candidates for further validation as diagnostic biomarkers for canine pancreatitis.
Keywords
Humans, Dogs, Animals, Mice, Circulating MicroRNA, Case-Control Studies, Retrospective Studies, MicroRNAs, Biomarkers, Pancreatitis, Chronic, Dog Diseases, Rodent Diseases, blood, diagnostics, gastrointestinal, liquid biopsy, transcriptomics
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Gastroenterology Commons, Medical Sciences Commons, Oncology Commons, Veterinary Medicine Commons
Comments
Supplementary Materials
PMID: 38351718