Faculty, Staff and Student Publications
Publication Date
12-20-2024
Journal
STAR Protocols
Abstract
Clonal hematopoiesis involves the clonal expansion of hematopoietic cells, potentially progressing into hematological malignancies. Here, we present a protocol for the development and characterization of two mouse models designed to simulate clonal hematopoiesis and acute myeloid leukemia. We describe steps for model generation, monitoring clonal expansion, harvesting, fixation, and staining of bone marrow and spleen tissues. We specifically focus on the visualization and analysis of p53 mutant clonal expansions, providing a comprehensive protocol for studying these phenomena in mouse models. For complete details on the use and execution of this protocol, please refer to Pourebrahim et al.
Keywords
Animals, Mice, Mice, Transgenic, Clonal Hematopoiesis, Disease Models, Animal, Tumor Suppressor Protein p53, Leukemia, Myeloid, Acute, Hematopoiesis, Hematopoietic Stem Cells, cell biology, flow cytometry, cancer, microscopy, model organisms
DOI
10.1016/j.xpro.2024.103362
PMID
39423122
PMCID
PMC11513565
PubMedCentral® Posted Date
10-17-2024
PubMedCentral® Full Text Version
Post-print
Graphical Abstract
Published Open-Access
yes
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Medical Sciences Commons, Oncology Commons