Faculty, Staff and Student Publications

Publication Date

12-20-2024

Journal

STAR Protocols

Abstract

Clonal hematopoiesis involves the clonal expansion of hematopoietic cells, potentially progressing into hematological malignancies. Here, we present a protocol for the development and characterization of two mouse models designed to simulate clonal hematopoiesis and acute myeloid leukemia. We describe steps for model generation, monitoring clonal expansion, harvesting, fixation, and staining of bone marrow and spleen tissues. We specifically focus on the visualization and analysis of p53 mutant clonal expansions, providing a comprehensive protocol for studying these phenomena in mouse models. For complete details on the use and execution of this protocol, please refer to Pourebrahim et al.

Keywords

Animals, Mice, Mice, Transgenic, Clonal Hematopoiesis, Disease Models, Animal, Tumor Suppressor Protein p53, Leukemia, Myeloid, Acute, Hematopoiesis, Hematopoietic Stem Cells, cell biology, flow cytometry, cancer, microscopy, model organisms

DOI

10.1016/j.xpro.2024.103362

PMID

39423122

PMCID

PMC11513565

PubMedCentral® Posted Date

10-17-2024

PubMedCentral® Full Text Version

Post-print

fx1.jpg (299 kB)
Graphical Abstract

Published Open-Access

yes

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