Faculty, Staff and Student Publications
Publication Date
5-1-2023
Journal
Molecular Oncology
Abstract
Accuracy and transparency of scientific data are becoming more and more relevant with the increasing concern regarding the evaluation of data reproducibility in many research areas. This concern is also true for quantifying coding and noncoding RNAs, with the remarkable increase in publications reporting RNA profiling and sequencing studies. To address the problem, we propose the following recommendations: (a) accurate documentation of experimental procedures in Materials and methods (and not only in the supplementary information, as many journals have a strict mandate for making Materials and methods as visible as possible in the main text); (b) submission of RT‐qPCR raw data for all experiments reported; and (c) adoption of a unified, simple format for submitted RT‐qPCR raw data. The Real‐time PCR Data Essential Spreadsheet Format (RDES) was created for this purpose.
Keywords
Humans, Reverse Transcriptase Polymerase Chain Reaction, Reproducibility of Results, RNA, Real-Time Polymerase Chain Reaction, accuracy, quantification, RNA, RT‐qPCR
DOI
10.1002/1878-0261.13418
PMID
36916500
PMCID
PMC10158759
PubMedCentral® Posted Date
3-27-2023
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Genetic Phenomena Commons, Medical Genetics Commons, Medical Molecular Biology Commons, Oncology Commons