
Faculty, Staff and Student Publications
Publication Date
10-7-2022
Journal
Science Advances
Abstract
Histone 2A (H2A) monoubiquitination is a fundamental epigenetics mechanism of gene expression, which plays a critical role in regulating cell fate. However, it is unknown if H2A ubiquitination is involved in EGFR-driven tumorigenesis. In the current study, we have characterized a previously unidentified oncogenic lncRNA (lncEPAT) that mediates the integration of the dysregulated EGFR pathway with H2A deubiquitination in tumorigenesis. LncEPAT was induced by the EGFR pathway, and high-level lncEPAT expression positively correlated with the glioma grade and predicted poor survival of glioma patients. Mass spectrometry analyses revealed that lncEPAT specifically interacted with deubiquitinase USP16. LncEPAT inhibited USP16's recruitment to chromatin, thereby blocking USP16-mediated H2A deubiquitination and repressing target gene expression, including
Keywords
Carcinogenesis, Chromatin, Clusterin, ErbB Receptors, Glioblastoma, Histones, Humans, RNA, Long Noncoding, Ubiquitin Thiolesterase
DOI
10.1126/sciadv.abn2571
PMID
36197973
PMCID
PMC9534510
PubMedCentral® Posted Date
10-5-2022
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Genetic Phenomena Commons, Medical Genetics Commons, Oncology Commons