Faculty, Staff and Student Publications
Publication Date
1-7-2025
Journal
Nucleic Acids Research
Abstract
Cell cycle checkpoints are the regulatory mechanisms that secure the strict order of cellular events for cell division that ensure genome integrity. It has been proposed that mitosis initiation depends on the completion of DNA replication, which must be tightly controlled to guarantee genome duplication. Contrary to these conventional hypotheses, we showed here that cells were able to enter mitosis without completion of DNA replication. Although DNA replication was not completed in cells upon depletion of MCM2, CDC45 or GINS4, these under-replicated cells progressed into mitosis, which led to cell death. These unexpected results challenge current model and suggest the absence of a cell cycle checkpoint that monitors the completion of DNA replication.
Keywords
Humans, DNA Replication, Mitosis, Cell Cycle Proteins, Chromosomal Proteins, Non-Histone, HeLa Cells, Cell Cycle, Minichromosome Maintenance Complex Component 2, Cell Cycle Checkpoints
DOI
10.1093/nar/gkae1311
PMID
39778868
PMCID
PMC11707533
PubMedCentral® Posted Date
1-8-2025
PubMedCentral® Full Text Version
Post-print
Graphical Abstract
Published Open-Access
yes
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Genetic Phenomena Commons, Medical Genetics Commons, Oncology Commons