Integrated Screens Uncover a Cell Surface Tumor Suppressor Gene KIRREL Involved in Hippo Pathway

Authors

Chao Wang
Xu Feng
Dan Su
Zhen Chen
Shimin Wang
Mengfan Tang
Min Huang
Litong Nie
Huimin Zhang
Siting Li
Ling Yin
Randy L Johnson
Traver Hart
Junjie Chen

Publication Date

6-21-2022

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abstract

Cell surface proteins play essential roles in various biological processes and are highly related to cancer development. They also serve as important markers for cell identity and targets for pharmacological intervention. Despite their great potentials in biomedical research, comprehensive functional analysis of cell surface proteins remains scarce. Here, with a de novo designed library targeting cell surface proteins, we performed in vivo CRISPR screens to evaluate the effects of cell surface proteins on tumor survival and proliferation. We found that

Keywords

Animals, Cell Proliferation, Genes, Tumor Suppressor, Hippo Signaling Pathway, Membrane Proteins, Mice, Neoplasms, Signal Transduction, Hippo, KIRREL, SAV1, CRISPR, integrated screens

DOI

10.1073/pnas.2121779119

PMID

35704761

PMCID

PMC9231494

PubMedCentral® Posted Date

6-15-2022

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes