Faculty, Staff and Student Publications
Publication Date
12-20-2024
Journal
STAR Protocols
Abstract
Herein, we present a protocol for culturing patient-derived organoids (PDOs) of cervical cancer that includes workflows for tumor biopsy/resection tissue and cytobrush-sampled cells. We describe steps for PDO culture initiation, including rinsing, gentle dissociation, Lymphoprep separation, and cell assessment, as well as seeding cells from surgical and cytobrush tissue digestion. We then provide guidance on PDO maintenance and passage and techniques for producing conditioned medium. Overall, this protocol serves as a valuable guide for establishing and maintaining cervical cancer PDOs. For complete details on the use and execution of this protocol, please refer to Colbert et al.
Keywords
Humans, Organoids, Uterine Cervical Neoplasms, Female, Cell Culture Techniques, Culture Media, Conditioned, Tumor Cells, Cultured
DOI
10.1016/j.xpro.2024.103353
PMID
39356641
PMCID
PMC11472619
PubMedCentral® Posted Date
10-1-2024
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Genetic Phenomena Commons, Medical Genetics Commons, Obstetrics and Gynecology Commons, Oncology Commons, Women's Health Commons