An In Vivo Microscopy Dataset for the Characterization of Leukocyte Death

Authors

Alain Pulfer
Diego Ulisse Pizzagalli
Miguel Palomino Segura
Nina Germic
Tommaso Virgilio
Mauro Di Pilato
Pau Carrillo Barbera
Elisa Palladino
Paola Antonello
Marcus Thelen
Hans-Uwe Simon
Rolf Krause
Santiago F Gonzalez

Publication Date

4-9-2025

Journal

Scientific Data

Abstract

Recent advancements in intravital microscopy have enabled the study of cell death in vivo under various experimental conditions, such as infection and cancer. However, the limited throughput of this technology, together with a lack of openly accessible datasets, affects the development of algorithms for the automatic detection and characterization of cell death, which in turn require the integration of extensive and curated datasets. To address these needs, we present a curated dataset of microscopy videos depicting the death of neutrophils, eosinophils, and dendritic cells, acquired in the spleen and in the lymph node of mice under inflammatory conditions. The dataset provides time-lapse imaging data, along with coordinates in space and time of cell death events displaying apoptotic-like morphodynamics, and 3D reconstruction of the cell morphology at each time point. Altogether, these data will be pivotal for developing computer vision and bioimage analysis methods to advance cell death research.

Keywords

Animals, Mice, Cell Death, Spleen, Leukocytes, Neutrophils, Intravital Microscopy, Lymph Nodes, Dendritic Cells, Eosinophils, Apoptosis, Time-Lapse Imaging

DOI

10.1038/s41597-025-04632-6

PMID

40204757

PMCID

PMC11982338

PubMedCentral® Posted Date

4-9-2025

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes