The Significant Impacts of Interleukin-8 Genotypes on the Risk of Colorectal Cancer in Taiwan

Authors

Chia-Wen Tsai
Wen-Shin Chang
Te-Cheng Yueh
Yun-Chi Wang
Yu-Ting Chin
Mei-Due Yang
Yi-Chih Hung
Mei-Chin Mong
Ya-Chen Yang
Jian Gu
Da-Tian Bau

Publication Date

10-10-2023

Journal

Cancers

Abstract

Interleukin-8 (IL-8), a pro-inflammatory cytokine, is upregulated in CRC and plays an important role in its development and progression. Genetic variants in the IL-8 gene may impact the risk of CRC by modulating IL-8 levels. Our primary objective was to investigate the role of IL-8 genotypes in the development of CRC. To accomplish this, we employed the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method to analyze the genotypes of IL-8 rs4017, rs2227306, rs2227543, and rs1126647 in 362 CRC patients and 362 controls. Additionally, we evaluated the interactions between these genotypes and factors such as age, gender, smoking, alcohol consumption, and body mass index (BMI) status in relation to the risk of CRC. Furthermore, we utilized quantitative reverse transcription-PCR to measure the serum IL-8. The results demonstrated a significant difference in the distribution of rs4017 genotypes between the control and case groups (p for trend = 0.0059). Logistic regression analysis revealed that individuals with variant AA genotype had a 1.92-fold higher CRC risk (95% confidence interval [CI] = 1.28-2.89, p = 0.0023). Moreover, carriers of the IL-8 rs4017 AT + AA genotypes exhibited a significant association with CRC risk (odds ratio [OR] = 1.39, 95% CI = 1.02-1.91, p = 0.0460). Additionally, individuals with IL-8 rs4017 AA genotype displayed significantly elevated serum IL-8 compared to those with TT genotype at a 1.73-fold level (p < 0.0001), indicating a correlation between genotype and phenotype. In conclusion, the genotypes of IL-8 rs4017, along with their associated expression levels, can potentially serve as predictive markers for the risk of CRC.

Keywords

colorectal cancer, genotype, IL-8, phenotype, polymorphism, Taiwan

DOI

10.3390/cancers15204921

PMID

37894288

PMCID

PMC10605288

PubMedCentral® Posted Date

10-10-2023

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes