Single-Cell RNA Sequencing Analysis Identifies Acute Changes in the Tumor Microenvironment Induced by Interferon α Gene Therapy in a Murine Bladder Cancer Model

Authors

Alexis R Steinmetz
Morgan Pierce
Alberto Martini
Come Tholomier
Ganiraju Manyam
Yan Chen
Akshay Sood
Jonathan J Duplisea
Burles A Johnson
Bogdan A Czerniak
Byron H Lee
Chinnaswamy Jagannath
Seppo Yla-Herttuala
Nigel R Parker
David J McConkey
Colin P Dinney
Sharada Mokkapati

Publication Date

1-1-2024

Journal

Frontiers in Immunology

Abstract

Introduction: Nadofaragene firadenovec (Ad-IFNα/Syn3) is now approved for BCG-unresponsive bladder cancer (BLCA). IFNα is a pleiotropic cytokine that causes direct tumor cell killing via TRAIL-mediated apoptosis, angiogenesis inhibition, and activation of the innate and adaptive immune system. We established an immunocompetent murine BLCA model to study the effects of murine adenoviral IFNα (muAd-Ifnα) gene therapy on cancer cells and the tumor microenvironment using a novel murine equivalent of Nadofaragene firadenovec (muAd-Ifnα).

Methods: Tumors were induced by instilling MB49 cells into the bladders of mice; luciferase imaging confirmed tumor development. Mice were treated with adenovirus control (Ad-Ctrl; empty vector), or muAd-Ifnα (3x1011 VP/mL), and survival analysis was performed. For single-cell sequencing (scRNAseq) analysis (72h), bladders were harvested and treated with collagenase/hyaluronidase and TrypLE for cell dissociation. Single cells were suspended in PBS/1% FBS buffer; viability was assessed with Vicell cell counter. scRNAseq analysis was performed using 10X genomics 3' sequencing. Raw RNAseq data were pre-processed using Cell Ranger single-cell software. Seurat (R package) was used to normalize and cluster the scRNA data. Pooled differential gene expression analysis in specific cell clusters was performed with DESeq2.

Results: We identified 16 cell clusters based on marker expression which were grouped into epithelial (tumor), uroplakin-enriched, endothelial, T-cells, neutrophils, and macrophage clusters. Top differentially expressed genes between muAd-Ifnα and Ad-Ctrl were identified. Within the specific cell clusters, IPA analysis revealed significant differences between muAd-Ifnα and control. IFNα signaling and hypercytokinemia/chemokinemia were upregulated in all clusters. Cell death pathways were upregulated in tumor and endothelial clusters. T-cells demonstrated upregulation of the immunogenic cell death signaling pathway and a decrease in the Th2 pathway genes. Macrophages showed upregulation of PD1/PD-L1 pathways along with downregulation of macrophage activation pathways (alternate and classical). Multiplex immunofluorescence confirmed increased infiltration with macrophages in muAd-Ifnα treated tumors compared to controls. PD1/PD-L1 expression was reduced at 72h.

Discussion: This single-cell analysis builds upon our understanding of the impact of Ad-IFNα on tumor cells and other compartments of the microenvironment. These data will help identify mechanisms to improve patient selection and therapeutic efficacy of Nadofaragene firadenovec.

Keywords

Animals, Urinary Bladder Neoplasms, Tumor Microenvironment, Mice, Single-Cell Analysis, Genetic Therapy, Interferon-alpha, Disease Models, Animal, Cell Line, Tumor, Adenoviridae, Mice, Inbred C57BL, Female

DOI

10.3389/fimmu.2024.1387229

PMID

39559365

PMCID

PMC11570268

PubMedCentral® Posted Date

11-4-2024

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes