Circulating Immune Signatures in Chronic Pancreatitis With and Without Preceding Acute Pancreatitis: A Pilot Study

Authors

Rasmus Hagn-Meincke
Dhiraj Yadav
Dana K Andersen
Santhi Swaroop Vege
Evan L Fogel
Jose Serrano
Melena D Bellin
Mark D Topazian
Darwin L Conwell
Liang Li
Stephen K Van Den Eeden
Asbjørn M Drewes
Stephen J Pandol
Chris E Forsmark
William E Fisher
Phil A Hart
Søren S Olesen
Walter G Park
Consortium for the Study of Chronic Pancreatitis, Diabetes, and Pancreatic Cancer (CPDPC)

Publication Date

5-1-2024

Journal

Pancreatology

Abstract

Objective: To investigate profiles of circulating immune signatures in healthy controls and chronic pancreatitis patients (CP) with and without a preceding history of acute pancreatitis (AP).

Methods: We performed a phase 1, cross-sectional analysis of prospectively collected serum samples from the PROspective Evaluation of Chronic Pancreatitis for EpidEmiologic and Translation StuDies (PROCEED) study. All samples were collected during a clinically quiescent phase. CP subjects were categorized into two subgroups based on preceding episode(s) of AP. Healthy controls were included for comparison. Blinded samples were analyzed using an 80-plex Luminex assay of cytokines, chemokines, and adhesion molecules. Group and pairwise comparisons of analytes were performed between the subgroups.

Results: In total, 133 patients with CP (111 with AP and 22 without AP) and 50 healthy controls were included. Among the 80 analytes studied, CP patients with a history of AP had significantly higher serum levels of pro-inflammatory cytokines (interleukin (IL)-6, IL-8, IL-1 receptor antagonist, IL-15) and chemokines (Cutaneous T-Cell Attracting Chemokine (CTACK), Monokine induced Gamma Interferon (MIG), Macrophage-derived Chemokine (MDC), Monocyte Chemoattractant Protein-1 (MCP-1)) compared to CP without preceding AP and controls. In contrast, CP patients without AP had immune profiles characterized by low systemic inflammation and downregulation of anti-inflammatory mediators, including IL-10.

Conclusion: CP patients with a preceding history of AP have signs of systemic inflammatory activity even during a clinically quiescent phase. In contrast, CP patients without a history of AP have low systemic inflammatory activity. These findings suggest the presence of two immunologically diverse subtypes of CP.

Keywords

Humans, Pilot Projects, Acute Disease, Cross-Sectional Studies, Cytokines, Pancreatitis, Chronic, Chemokines, Interleukin-6, Immune signatures, Chronic Pancreatitis

DOI

10.1016/j.pan.2024.02.012

PMID

38461145

PMCID

PMC11023786

PubMedCentral® Posted Date

5-1-2025

PubMedCentral® Full Text Version

Author MSS

Published Open-Access

yes