Ampk Regulates Homeostasis of Invasion and Viability in Trophoblasts by Redirecting Glucose Metabolism: Implications for Pre-eclampsia

Authors

Ping Xu
Yangxi Zheng
Jiujiang Liao
Mingyu Hu
Yike Yang
Baozhen Zhang
Mark D Kilby
Huijia Fu
Yamin Liu
Fumei Zhang
Liling Xiong
Xiyao Liu
Huili Jin
Yue Wu
Jiayu Huang
Tingli Han
Li Wen
Rufei Gao
Yong Fu
Xiujun Fan
Hongbo Qi
Philip N Baker
Chao Tong

Publication Date

2-1-2023

Journal

Cell Proliferation

Abstract

Pre-eclampsia (PE) is deemed an ischemia-induced metabolic disorder of the placenta due to defective invasion of trophoblasts during placentation; thus, the driving role of metabolism in PE pathogenesis is largely ignored. Since trophoblasts undergo substantial glycolysis, this study aimed to investigate its function and regulatory mechanism by AMPK in PE development. Metabolomics analysis of PE placentas was performed by gas chromatography-mass spectrometry (GC-MS). Trophoblast-specific AMPKα1-deficient mouse placentas were generated to assess morphology. A mouse PE model was established by Reduced Uterine Perfusion Pressure, and placental AMPK was modulated by nanoparticle-delivered A769662. Trophoblast glucose uptake was measured by 2-NBDG and 2-deoxy-d-[

Keywords

Humans, Mice, Animals, Pregnancy, Female, Trophoblasts, Placenta, AMP-Activated Protein Kinases, Pre-Eclampsia, Homeostasis, Glucose, Cell Movement

DOI

10.1111/cpr.13358

PMID

36480593

PMCID

PMC9890534

PubMedCentral® Posted Date

12-8-2022

PubMedCentral® Full Text Version

Post-print

Published Open-Access

yes