Student and Faculty Publications
Publication Date
12-16-2022
Journal
STAR Protocols
Abstract
Drug testing assays in hematopoietic stem and progenitor cells (HSPCs) are fundamental in biological studies of myelodysplastic syndromes (MDS) but have historically entailed a technical challenge. This protocol allows the efficient isolation of MDS HSPCs from bone marrow mononuclear cell fractions and their culturing with the support of stromal cells for improved maintenance during drug testing. Lastly, specific steps are given to quantify surviving cells and assess changes in the HSPC hierarchies.
For complete details on the use and execution of this protocol, please refer to Ganan-Gomez et al. (2022).
Keywords
Humans, Bone Marrow, Hematopoietic Stem Cells, Myelodysplastic Syndromes, Stromal Cells, Bone Marrow Cells
Included in
Bioinformatics Commons, Biomedical Informatics Commons, Medical Sciences Commons, Oncology Commons
Comments
PMID: 36240061