Faculty, Staff and Student Publications
Publication Date
1-1-2010
Journal
Methods in Molecular Biology
Abstract
A protocol is described using lipid mutants and thiol-specific chemical reagents to study lipid-dependent and host-specific membrane protein topogenesis by the substituted-cysteine accessibility method as applied to transmembrane domains (SCAM). SCAM is adapted to follow changes in membrane protein topology as a function of changes in membrane lipid composition. The strategy described can be adapted to any membrane system.
Keywords
Bacterial Proteins, Blotting, Western, Electrophoresis, Polyacrylamide Gel, Glycosyltransferases, Immunoprecipitation, Membrane Lipids, Membrane Proteins, Membrane Transport Proteins, Phosphatidylethanolamines, Phospholipids
DOI
10.1007/978-1-60327-412-8_5
PMID
I20419405
PMCID
PMC3099133
PubMedCentral® Posted Date
May 2011
PubMedCentral® Full Text Version
Author MSS
Published Open-Access
yes