Faculty, Staff and Student Publications
Publication Date
6-1-2025
Journal
FEBS Open Bio
Abstract
AMP-activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in modulating metabolism and other cellular processes. Substantial efforts have been made to develop pharmacological modulators of AMPK activity due to their therapeutic potential against various diseases. Measuring AMPK activity in vitro is a fundamental step for testing AMPK activators and inhibitors. Here, we report an enzyme-linked immunosorbent assay (ELISA)-based AMPK activity assay with simple steps and high sensitivity. This assay offers a robust, in-house alternative to the traditional radioactive methods and other approaches that rely on specialized reagents or commercial kits.
Keywords
AMP-Activated Protein Kinases, Enzyme-Linked Immunosorbent Assay, Humans, AMPK, ELISA, kinase activity
DOI
10.1002/2211-5463.70017
PMID
40066852
PMCID
PMC12127874
PubMedCentral® Posted Date
3-11-2025
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes