Faculty, Staff and Student Publications
Publication Date
7-1-2024
Journal
Arteriosclerosis, Thrombosis, and Vascular Biology
Abstract
The implementation of human induced pluripotent stem cell (hiPSC) models has introduced an additional tool for identifying molecular mechanisms of disease that complement animal models. Patient-derived or CRISPR/Cas9-edited induced pluripotent stem cells differentiated into smooth muscle cells (SMCs) have been leveraged to discover novel mechanisms, screen potential therapeutic strategies, and model in vivo development. The field has evolved over almost 15 years of research using hiPSC-SMCs and has made significant strides toward overcoming initial challenges such as the lineage specificity of SMC phenotypes. However, challenges both specific (eg, the lack of specific markers to thoroughly validate hiPSC-SMCs) and general (eg, a lack of transparency and consensus around methodology in the field) remain. In this review, we highlight the recent successes and remaining challenges of the hiPSC-SMC model.
Keywords
Humans, Induced Pluripotent Stem Cells, Myocytes, Smooth Muscle, Cell Differentiation, Animals, Phenotype, Muscle, Smooth, Vascular, Cell Lineage, cell culture techniques, three dimensional, cell lineage, induced pluripotent stem cells, myocytes, smooth muscle, vascular diseases
DOI
10.1161/ATVBAHA.123.319703
PMID
38695171
PMCID
PMC11209779
PubMedCentral® Posted Date
7-1-2025
PubMedCentral® Full Text Version
Post-print
Published Open-Access
yes