Faculty, Staff and Student Publications
Publication Date
1-1-2024
Journal
Biotechnology Progress
Abstract
Initiating and regulating humoral immunity, Fc gamma receptors (FcγRs) have been identified both as therapeutics and as drug targets, and thus production of biologically active FcγRs is highly demanded for biopharmaceutical development. Focusing on low-affinity FcγRs IIA (131H/R allotypes), IIB, and IIIA (176F/V), this study used human 293-F cells to achieve correct post-translational modifications (PTMs) including biotinylation, N-glycosylation, and disulfides. Approaches involving co-expression of FcγR-AviTag and Escherichia coli biotin ligase BirA, endoplasmic reticulum retention, stable and transient transfections, and optimization of transgene ratio were investigated. Protein electrophoresis under reducing and non-reducing conditions, enzymatic deglycosylation, streptavidin pull-down assays, and binding kinetic analysis collectively indicated that the produced FcγR ectodomains were fully biotinylated, N-glycosylated, had formed disulfide bond, and exhibited expected binding affinities toward IgG1 trastuzumab and its Fc mutants. A clear trade-off between production yield and PTM quality was also observed. Achieving multiple types of PTMs completely by one-step cell culture should have applications for the production of a variety of complex proteins of biomedical importance.
Keywords
Humans, Receptors, IgG, Glycosylation, Kinetics, Immunoglobulin G, Escherichia coli, FcγR, glycosylation, biotinylation, PTM
DOI
10.1002/btpr.3392
PMID
37734055
PMCID
PMC10922510
PubMedCentral® Posted Date
1-1-2025
PubMedCentral® Full Text Version
Author MSS
Published Open-Access
yes