Genetic Variations Mir-10Aa>T, Mir-30Ca>G, Mir-181At>C, and Mir-499Ba>G and the Risk of Recurrent Pregnancy Loss in Korean Women

Authors

Hui-Jeong An
Sung-Hwan Cho
Han-Sung Park
Ji-Hyang Kim
Young-Ran Kim
Woo-Sik Lee
Jung-Ryeol Lee
Seong-Soo Joo
Eun-Hee Ahn
Nam-Keun Kim

Publication Date

9-25-2022

Journal

Biomedicines

Abstract

This study investigated the genetic association between recurrent pregnancy loss (RPL) and microRNA (miRNA) polymorphisms in miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G in Korean women. Blood samples were collected from 381 RPL patients and 281 control participants, and genotyping of miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G was carried out by TaqMan miRNA RT-Real Time polymerase chain reaction (PCR). Four polymorphisms were identified, including miR-10aA>T, miR-30cA>G, miR-181aT>C, and miR-499bA>G. MiR-10a dominant model (AA vs. AT + TT) and miR-499bGG genotypes were associated with increased RPL risk (adjusted odds ratio [AOR] = 1.520, 95% confidence interval [CI] = 1.038−2.227, p = 0.032; AOR = 2.956, 95% CI = 1.168−7.482, p = 0.022, respectively). Additionally, both miR-499 dominant (AA vs. AG + GG) and recessive (AA + AG vs. GG) models were significantly associated with increased RPL risk (AOR = 1.465, 95% CI = 1.062−2.020, p = 0.020; AOR = 2.677, 95% CI = 1.066−6.725, p = 0.036, respectively). We further propose that miR-10aA>T, miR-30cA>G, and miR-499bA>G polymorphisms effects could contribute to RPL and should be considered during RPL patient evaluation.

Keywords

microRNA, recurrent pregnancy loss, single-nucleotide polymorphism (SNP)

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