Language
English
Publication Date
5-22-2026
Journal
bioRxiv
DOI
10.64898/2026.05.21.726738
PMID
42239467
PMCID
PMC13228616
PubMedCentral® Posted Date
5-22-2026
PubMedCentral® Full Text Version
Pre-Print
Abstract
Biomolecular condensates concentrate RNA and protein machinery that facilitate RNA processing, ribonucleoprotein assembly, and gene regulation. Some evidence supports that condensates can modulate RNA folding, but measuring RNA structure within native condensates remains an unsolved challenge. Here we introduce RAID-MaP, a strategy that combines APEX proximity labeling with dimethyl sulfate (DMS) chemical probing to measure RNA structure within defined subcellular compartments. We applied RAID-MaP to resolve late stages of ribosomal RNA (rRNA) folding within the granular component (GC) of the nucleolus, revealing that both the 18S and 28S rRNAs feature widespread differences in structure compared to assembled ribosomes consistent with ongoing folding of both secondary and tertiary structure. We further combined RAID-MaP with transcription inhibition to resolve kinetics of rRNA maturation. The maturation kinetics of both subunits progressed on comparable timescales but with characteristic domain-level ordering, with late GC-resident intermediates often becoming more protected than mature ribosomes suggestive of stabilization by nucleolar accessory factors. Perturbing this 28S assembly pathway using antisense oligonucleotides produces distinct nucleolar phenotypes depending on whether early versus late folding domains are disrupted, demonstrating a direct link between rRNA folding and phase separation. We additionally applied RAID-MaP to discover that the 7SK small nuclear RNA undergoes spatially regulated structural switching consistent with localized release of the transcription factor P-TEFb at sites of active transcription. Together, our results establish subcellular spatial control of RNA structure as a new dimension of RNA regulation.
Published Open-Access
yes
Recommended Citation
Bose, Ritwika; Bergstrom, Erik W; Taylor, Sovanny R; et al., "Mapping RNA Structure Assembly and Remodeling in Biomolecular Condensates" (2026). Faculty, Staff and Students Publications. 8188.
https://digitalcommons.library.tmc.edu/baylor_docs/8188