Candidate Circulating microRNA Biomarkers in Dogs With Chronic Pancreatitis

Authors

Susan K Armstrong
Robert W Hunter
Wilna Oosthyuzen
Maciej Parys
Adam G Gow
Silke Salavati Schmitz
James W Dear
Richard J Mellanby

Publication Date

1-1-2024

Journal

Journal of Veterinary Internal Medicine

Abstract

BACKGROUND: Pancreatitis is an important cause of disease and death in dogs. Available circulating biomarkers are not sufficiently sensitive and specific for a definitive diagnosis.

HYPOTHESIS: Circulating microRNAs would be differentially expressed in dogs with chronic pancreatitis and could have potential as diagnostic biomarkers.

ANIMALS: Healthy controls (n = 19) and dogs with naturally occurring pancreatitis (n = 17).

METHODS: A retrospective case-control study. Dogs with pancreatitis were included if they satisfied diagnostic criteria for pancreatitis as adjudicated by 3 experts. MicroRNA was extracted from stored serum samples and sequenced. Reads were mapped to mature microRNA sequences in the canine, mouse, and human genomes. Differentially expressed microRNAs were identified and the potential mechanistic relevance explored using Qiagen Ingenuity Pathway Analysis (IPA).

RESULTS: Reads mapping to 196 mature microRNA sequences were detected. Eight circulating microRNAs were significantly differentially expressed in dogs with pancreatitis (≥2-fold change and false discovery rate

CONCLUSIONS: The significantly differentially expressed microRNAs represent promising candidates for further validation as diagnostic biomarkers for canine pancreatitis.

Keywords

Humans, Dogs, Animals, Mice, Circulating MicroRNA, Case-Control Studies, Retrospective Studies, MicroRNAs, Biomarkers, Pancreatitis, Chronic, Dog Diseases, Rodent Diseases, blood, diagnostics, gastrointestinal, liquid biopsy, transcriptomics

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Supplementary Materials

PMID: 38351718