Processing and Cryopreservation of Human Ureter Tissues for Single-Cell and Spatial Transcriptomics Assays

Authors

Emily E Fink
Surbhi Sona
Byron H Lee
Angela H Ting

Publication Date

12-16-2022

Journal

STAR Protocols

Abstract

Characterizing the cellular heterogeneity of human ureter tissues using single-cell RNA sequencing (scRNA-seq) and spatial transcriptomics provides a detailed atlas of cell types, signaling networks, and potential cell-cell cross talk underlying developmental and regenerative pathways. We describe an optimized protocol for generating, cryopreserving, and thawing single-cell suspensions from ureter tissues isolated post-cystectomy for scRNA-seq. In addition, we describe an optimized protocol for cryopreserving human ureter tissues for 10x Genomics Visium spatial gene expression platform. For complete details on the use and execution of this protocol, please refer to Fink et al. (2022).

Keywords

Humans, Transcriptome, Ureter, Cryopreservation, Gene Expression Profiling, Biological Assay, Cell isolation, Single Cell

Comments

Data Availability Statement

PMID: 36595885