Inhibition of Colorectal Cancer Tumorigenesis by Ursolic Acid and Doxorubicin Is Mediated by Targeting the Akt Signaling Pathway and Activating the Hippo Signaling Pathway

Authors

Dan Hu
Ruo Yu Meng
Thi Van Nguyen
Ok Hee Chai
Byung Hyun Park
Ju-Seog Lee
Soo Mi Kim

Publication Date

1-27-2023

Journal

Molecular Medicine Reports

Abstract

Colorectal cancer (CRC) is one of the deadliest malignant tumors worldwide and its prevalence is increasing in South Korea. The efficacy of combined treatment with natural product‑derived and chemotherapy agents including curcumin combined with 5‑fluorouracil, resveratrol combined with cisplatin and epigallocatechin‑3‑gallate (EGCG) combined with cisplatin in preventing cancer progression and killing cancer cells has emerged. The Akt and Hippo signaling pathways serve a key role in colorectal tumor growth; however, the exact role of the crosstalk between Akt and Hippo signaling pathways in CRC remains poorly elucidated. The combined effect of UA and DOX on the cell proliferation, apoptosis, migration and cell cycle of CRC cells were investigated by performing Cell proliferation assay, a soft agar colony formation assay, flow cytometry, wound healing assay and western blotting assay. Subsequently, the expression of AKT and Hippo signaling pathway‑associated proteins were also assessed by western blot assay. Moreover, a xenograft nude mouse model was constructed to verify the effects of UA and DOX on the tumorigenesis of HCT116 cell

Keywords

Humans, Mice, Animals, Phosphatidylinositol 3-Kinases, Proto-Oncogene Proteins c-akt, Hippo Signaling Pathway, Cisplatin, Glycogen Synthase Kinase 3 beta, Xenograft Model Antitumor Assays, Signal Transduction, Carcinogenesis, Cell Proliferation, Apoptosis, Doxorubicin, Colorectal Neoplasms, Mammals, Ursolic Acid

Comments

Supplementary Materials

Data Availability Statement

PMID: 36382656