AIBP Regulates TRPV1 Activation in Chemotherapy-Induced Peripheral Neuropathy by Controlling Lipid Raft Dynamics and Proximity to TLR4 in Dorsal Root Ganglion Neurons

Authors

Juliana M Navia-Pelaez
Julia Borges Paes Lemes
Leonardo Gonzalez
Lauriane Delay
Luciano Dos Santos Aggum Capettini
Jenny W Lu
Gilson Gonçalves Dos Santos
Ann M Gregus
Patrick M Dougherty
Tony L Yaksh
Yury I Miller

Publication Date

6-1-2023

Journal

Pain

Abstract

Nociceptive afferent signaling evoked by inflammation and nerve injury is mediated by the opening of ligand-gated and voltage-gated receptors or channels localized to cholesterol-rich lipid raft membrane domains. Dorsal root ganglion (DRG) nociceptors express high levels of toll-like receptor 4 (TLR4), which also localize to lipid rafts. Genetic deletion or pharmacologic blocking of TLR4 diminishes pain associated with chemotherapy-induced peripheral neuropathy (CIPN). In DRGs of mice with paclitaxel-induced CIPN, we analyzed DRG neuronal lipid rafts, expression of TLR4, activation of transient receptor potential cation channel subfamily V member 1 (TRPV1), and TLR4-TRPV1 interaction. Using proximity ligation assay, flow cytometry, and whole-mount DRG microscopy, we found that CIPN increased DRG neuronal lipid rafts and TLR4 expression. These effects were reversed by intrathecal injection of apolipoprotein A-I binding protein (AIBP), a protein that binds to TLR4 and specifically targets cholesterol depletion from TLR4-expressing cells. Chemotherapy-induced peripheral neuropathy increased TRPV1 phosphorylation, localization to neuronal lipid rafts, and proximity to TLR4. These effects were also reversed by AIBP treatment. Regulation of TRPV1-TLR4 interactions and their associated lipid rafts by AIBP covaried with the enduring reversal of mechanical allodynia otherwise observed in CIPN. In addition, AIBP reduced intracellular calcium in response to the TRPV1 agonist capsaicin, which was increased in DRG neurons from paclitaxel-treated mice and in the naïve mouse DRG neurons incubated in vitro with paclitaxel. Together, these results suggest that the assembly of nociceptive and inflammatory receptors in the environment of lipid rafts regulates nociceptive signaling in DRG neurons and that AIBP can control lipid raft-associated nociceptive processing.

Keywords

Animals, Mice, Rats, Antineoplastic Agents, Carrier Proteins, Cholesterol, Ganglia, Spinal, Membrane Microdomains, Neurons, Paclitaxel, Peripheral Nervous System Diseases, Rats, Sprague-Dawley, Toll-Like Receptor 4, TRPV Cation Channels

Comments

Supplementary Materials

PMID: 36719418